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btc radio vol 23 may 2018
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For diagnosis, tissue should be obtained for both culture and histopathology. The patient was submitted to a thoracotomy, and a wedge pulmonary resection was performed. The collections of macrophages distort the organ architecture and produce necrotic areas. Histopathology still appears to be the best screening tool to define the presence of fungal elements and verify that the fungus is causing disease in the tissue 19 ; however, to define the specific fungus or fungi present, nucleic acid detection PCR or in situ hybridization is probably more specific than immunohistochemistry at this time. Tissue identification of these molds is a very important diagnostic tool, since it distinguishes the presence of the fungus as a pathogen in the specimen from a culture contaminant and is indispensable to define whether there is blood vessel invasion.
